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成分（g/L）
| Pancreatic Digest of Gelatin/明胶蛋白胨 |
10.0 |
| Glucose monohydrate/葡萄糖 | 5.0 |
| Disodium hydrogen phosphate dihydrate/磷酸氢二钠 | 8.0 |
| Potassium dihydrogen phosphate/磷酸二氢钾 |
2.0 |
| Dehydrated Ox Bile/牛胆盐 | 20.0 |
| Brilliant Green/煌绿 |
0.015 |
| pH值7.2±0.2 |
25℃ |
用 法：Suspend 45.0g/liter, and heat toboilingfor 1 to 2 minutes.Transfer 120-ml portionsto 250-ml volumetric flasks or9-ml portions to test tubes,all being capped with cotton plugs or loose-ftting caps.Heat at 100℃ for 30 min and cool immediately.
称取本品45.0g,加热搅拌溶解于1000ml蒸馏水中，分装三角瓶每瓶120ml或分装试管,每管9ml,100℃水浴加热30分钟，迅速在流动的冷水中冷却，无需高压灭菌。